<\/span><\/h2>\nWhat are the different ways to order custom DNA oligonucleotides?<\/h3>\n
You can order custom DNA oligonucleotides using three methods: manual entry (specifying all parameters including name, sequence, modifications, purification, scale, and delivery), copy and paste (of the name and sequence, using specified modification designators), and file upload (using a provided Excel template).<\/p>\n
What are the specifications for Express Oligos?<\/h3>\n
Express Oligos offer same-day synthesis and shipping within the US. To qualify, sequences must be 5-40 nucleotides long, orders must be placed by 5:00 pm ET (Monday-Friday), only 10 or 25 nmole scales are available with “None, Ship Entire Yield Dry” selected for normalization and shipping, next-day shipping is mandatory, only standard DNA bases (A, C, G, T) are permitted (no modifications or wobbles), and a maximum of 25 express oligos can be ordered per day.<\/p>\n
Can I order oligonucleotides with modifications?<\/h3>\n
Yes, modifications are available for standard orders but are not permitted for Express Oligos. You can specify 5\u2032, 3\u2032, or internal modifications during the ordering process for standard oligos.<\/p>\n
What purification methods are available?<\/h3>\n
The available purification methods vary depending on the chosen format (Value or Standard) and may include desalting. More details can be found in the ordering tool.<\/p>\n
What are the yield guarantees for my oligos?<\/h3>\n
Yield guarantees depend on the oligo’s length, the amount ordered (nmoles or \u00b5moles), and whether the oligo is modified. For unmodified oligos between 25 and 50 bases, specific yield guarantees are listed in our documentation. These guarantees may vary for oligos outside this length range. Actual yields for other oligos may vary.<\/p>\n
What happens if my Express Oligo order fails quality control?<\/h3>\n
If your Express Oligo order fails quality control (due to factors like high degenerate base percentages, low yields, or GC-rich sequences), same-day shipping will not be possible.<\/p>\n
How can I check if my sequence is eligible for Express Oligo synthesis?<\/h3>\n
You can check eligibility by entering your sequence in the online order editor. A green message will confirm eligibility.<\/p>\n
Can I use degenerate bases in my order?<\/h3>\n
Yes, you can order oligonucleotides with degenerate bases using IUB codes (e.g., B=C+G+T, D=A+G+T, H=A+C+T, K=T+G, M=A+C, N=A+C+G+T) at no extra charge for standard orders. The synthesizer will incorporate equal quantities of the specified bases. Degenerate bases are not allowed in Express Oligo orders.<\/p>\n
What quality control measures are implemented?<\/h3>\n
All oligos undergo deprotection and desalting. Standard oligos undergo ESI-MS (Electrospray Ionization Mass Spectrometry) analysis, except for mixed-base oligos. All oligos also undergo dual UV spectrophotometry quantification for accurate yield determination.<\/p>\n
What is the difference between Value and Standard formats?<\/h3>\n
The Value format is most economical and fastest, ideal for smaller-scale projects (25 or 50 nmol scale). The Standard format is for larger scales and accommodates various formats beyond tubes, allowing for 5\u2032, 3\u2032, or internal modifications. More details are available on our website.<\/p>\n
What happens if I need to cancel my order?<\/h3>\n
Express Oligo orders cannot be canceled. Standard orders may have cancellation policies detailed in our terms and conditions.<\/p>\n
What scale sizes are available for ordering?<\/h3>\n
Available scale sizes depend on the format (Value or Standard) and whether you are ordering Express Oligos. Options include 10, 25, 50, 100, 250 nmoles, and \u00b5mole scales for standard orders. Express Oligo orders are limited to 10 or 25 nmole scales.<\/p>\n
How long will it take to receive my order?<\/h3>\n
Express Oligo orders within the US, meeting all criteria, are delivered the same day. Standard orders have varying turnaround times depending on the scale and other factors.<\/p>\n
What is the maximum number of oligos I can order on Express Oligo in a day?<\/h3>\n
You can order a maximum of 25 Express Oligos per day.<\/p>\n
What is the length range for oligos I can order?<\/h3>\n
The length range varies depending on the order type. Express Oligos must be between 5 and 40 nucleotides long. Standard Oligos have a broader length range; see detailed specifications on the website.<\/p>\n","protected":false},"excerpt":{"rendered":"
Designing and ordering custom DNA oligonucleotides can seem daunting, but understanding the process, particularly the nuances of primer order, is key to successful research. This article will break down the key aspects of ordering custom oligos, focusing on factors that influence the yield, quality, and speed of delivery. Primer Order: Factors Affecting Yield and Quality…<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[9],"tags":[],"class_list":["post-1829","post","type-post","status-publish","format-standard","hentry","category-blog"],"_links":{"self":[{"href":"https:\/\/leeresumen.com\/social\/wp-json\/wp\/v2\/posts\/1829","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/leeresumen.com\/social\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/leeresumen.com\/social\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/leeresumen.com\/social\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/leeresumen.com\/social\/wp-json\/wp\/v2\/comments?post=1829"}],"version-history":[{"count":1,"href":"https:\/\/leeresumen.com\/social\/wp-json\/wp\/v2\/posts\/1829\/revisions"}],"predecessor-version":[{"id":3198,"href":"https:\/\/leeresumen.com\/social\/wp-json\/wp\/v2\/posts\/1829\/revisions\/3198"}],"wp:attachment":[{"href":"https:\/\/leeresumen.com\/social\/wp-json\/wp\/v2\/media?parent=1829"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/leeresumen.com\/social\/wp-json\/wp\/v2\/categories?post=1829"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/leeresumen.com\/social\/wp-json\/wp\/v2\/tags?post=1829"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
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